Publications

2023

Oliveira, A. R., Ramou, E., Palma, S. I. C. J., Esteves, C., Barbosa, A. & Roque, A. C. A. (2023). Impact of the cationic moiety of ionic liquids on chemoselective artificial olfaction. ACS Mater. Au, 3, 678-686. Website

Dias, A. M. G. C., Moreira, I. P., Lychko, I., Soares, C. L., Nurrito, A., Barbosa, A. J. M., Lutz-Bueno, V., Mezzenga, R., Carvalho, A. L., Pina, A. S. & Roque, A. C. A. (2023). Hierarchical self-assembly of a reflectin-derived peptide. Front. Chem., 11. Website

Lychko, I., Soares, C. L., Dias, A. M. G. C. & Roque, A. C. A. (2023). A scalable method to purify reflectins from inclusion bodies. Sep. Purif. Technol., 315, 123736. Website

Oliveira, A. R., Costa, H. M. A., Ramou, E., Palma, S. I. C. J., & Roque, A. C. A. (2023). Effect of polymer hydrophobicity in the performance of hybrid gel gas sensors for e-noses. Sensors, 23, 1-18. Website

Ramou, E., & Roque, A. C. A. (2023). Textural landscapes of VOC-sensitive chiral liquid crystal-based materials. Applied Physics Reviews, 10(February), 011411. Website

Ramou, E., Palma, S. I. C. J., & Roque, A. C. A. (2023). A room temperature 9CB-based chemical sensor. Nano Select, 1–12. Website

2022

Dias, A. M. G. C., Cena, C., Lutz-bueno, V., Mezzenga, R., Marques, A., Ferreira, I., & Roque, A. C. A. (2022). Solvent modulation in peptide sub-microfibers obtained by solution blow spinning. Frontiers in Chemistry, 10(December), 1–9. Website

Costa, C. F. S., Barbosa, A. J. M., Dias, A. M. G. C., & Roque, A. C. A. (2022). Native, engineered and de novo designed ligands targeting the SARS-CoV-2 spike protein. Biotechnology Advances, 59(April), 1–12. Website

Esteves, C., Palma, S. I. C. J., Costa, H. M. A., Alves, C., Santos, G. M. C., Ramou, E., Carvalho, A. L., Alves, V., & Roque, A. C. A. (2022). Tackling Humidity with Designer Ionic Liquid-Based Gas Sensing Soft Materials. Advanced Materials, 34(8), 1–14. PDF

Moreira, I. P., Esteves, C., Palma, S. I. C. J., Ramou, E., Carvalho, A. L. M., & Roque, A. C. A. (2022). Synergy between silk fibroin and ionic liquids for active gas-sensing materials. Materials Today Bio, 15(May). Website

Pina, Ana S., Morgado, L., Duncan, K. L., Carvalho, S., Carvalho, H. F., Barbosa, A. J. M., de P. Mariz, B., Moreira, I. P., Kalafatovic, D., Morais Faustino, B. M., Narang, V., Wang, T., Pappas, C. G., Ferreira, I., Roque, A. C. A., & Ulijn, R. V. (2022). Discovery of phosphotyrosine-binding oligopeptides with supramolecular target selectivity. Chemical Science, 13(1), 210–217. Website

Ramou, E., Palma, S. I. C. J., & Roque, A. C. A. (2022). Nanoscale Events on Cyanobiphenyl-Based Self-Assembled Droplets Triggered by Gas Analytes. ACS Applied Materials and Interfaces, 14(4), 6261–6273. PDF

Reviewed conference papers

Santos, J. J. M., Palma, S. I. C. J., Esteves, C., Gamboa, H., Oliveira, J.P., & Roque, A. C. A. (2022). An electrical model characterization of an electronic nose chemical sensor using a programmable system-on-a-chip based AFE. 2022 International Young Engineers Forum (YEF-ECE), 33-38. Website 

Alves, R., Rodrigues, J., Ramou, E., Palma, S., Roque, A., & Gamboa, H. (2022). Classification of Volatile Compounds with Morphological Analysis of e-nose Response. Proceedings of the 15th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2022), 4, 31–39. PDF

Esteves, C., Palma, S., Costa, H., Alves, C., Santos, G., Ramou, E., & Roque, A. C. (2022). VOC Sensing in Humid and Dry Environments. International Symposium on Olfaction and Electronic Nose, ISOEN 2022 – Proceedings, 1–3. PDF

Palma, S. I. C. J., Frazao, J., Alves, R., Costa, H. M. A., Alves, C., Gamboa, H., Silveira, M., Roque, A. C. A. (2022). Learning to see VOCs with Liquid Crystal Droplets. International Symposium on Olfaction and Electronic Nose, ISOEN 2022 – Proceedings, 56–59. PDF  Website

Oliveira, A., Ramou, E., Teixeira, G., Palma, S., & Roque, A. (2022). Incorporation of VOC-Selective Peptides in Gas Sensing Materials. Proceedings of the 15th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2022), 1(Biostec), 25–34. PDF Website

2021

dos Santos, R., Romão, M. J., Roque, A. C. A., & Carvalho, A. L. (2021). Magnetic particles used in a new approach for designed protein crystallization. CrystEngComm, 23(5), 1083–1090. Website

Fernandes, C. S. M., Pina, A. S., & Roque, A. C. A. (2021). Affinity-triggered hydrogels: Developments and prospects in biomaterials science. Biomaterials, 268. Website

Frazão, J. é. L., Palma, S. I. C. J., Costa, H. M. A., Alves, C., Roque, A. C. A., & Silveira, M. (2021). Optical Gas Sensing with Liquid Crystal Droplets and Convolutional Neural Networks. Sensors, 21(8), 1–15. PDF

Gonçalves, W. B., Cervantes, E. P., Pádua, A. C. C. S., Santos, G., Palma, S. I. C. J., Li, R. W. C., Roque, A. C. A., & Gruber, J. (2021). Ionogels Based on a Single Ionic Liquid for Electronic Nose Application. Chemosensors, 9(8), 1–13. PDF Website

Matos, M. J.B., Trovão, F., Gonçalves, J., Rothbauer, U., Freire, M. G., Barbosa, A. M. J. B., Pina, A. S., & Roque, A. C. A. (2021). A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent. Separation and Purification Technology, 265, 1–10. Website

Ramou, E., Rebordão, G., Palma, S. I. C. J., & Roque, A. C. A. (2021). Stable and Oriented Liquid Crystal Droplets Stabilized by Imidazolium Ionic Liquids. Molecules, 26(19), 1–15. PDF

Mariz, B. de P., Carvalho, S., Batalha, I. L., & Pina, A. S. (2021). Artificial enzymes bringing together computational design and directed evolution. Organic and Biomolecular Chemistry, 19(9), 1915–1925. Website

Reviewed conference papers

Moreira, I. P., Sato, L., Alves, C., Palma, S., & Roque, A. C. (2021). Fish Gelatin-based Films for Gas Sensing. Proceedings of the 14th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2021), 1(Biostec), 32–39. PDF

Book chapters

Pina, A. S., Batalha, Í. L., Dias, A. M. G. C., & Roque, A. C. A. (2021). Affinity Tags in Protein Purification and Peptide Enrichment: An Overview. In N. E. Labrou (Ed.), Protein Downstream Processing: Design, Development, and Application of High and Low-Resolution Methods (2nd ed., Vol. 2178, pp. 107–132). New York: Humana Press.

2020

Esteves, C., Ramou, E., Porteira, A. R. P., Barbosa, A. J. M., & Roque, A. C. A. (2020). Seeing the Unseen: The Role of Liquid Crystals in Gas-Sensing Technologies. Advanced Optical Materials, 8(11), 1–29. PDF

Fernandes, C. S. M., Rodrigues, A. L., Alves, V. D., Fernandes, T. G., Pina, A. S., & Roque, A. C. A. (2020). Natural Multimerization Rules the Performance of Affinity-Based Physical Hydrogels for Stem Cell Encapsulation and Differentiation. Biomacromolecules, 21(8), 3081–3091. Website

Matos, Manuel J.B., Pina, A. S., & Roque, A. C. A. (2020). Rational design of affinity ligands for bioseparation. Journal of Chromatography A, 1619, 1–14. Website

Padrão, I., Fernandes, C. S. M., Esteves, C., Fernandes, T., Pina, A. S., & Roque, A. C. A. (2020). Versatile and Tunable Poly(Ethylene Glycol)-Based Hydrogels Crosslinked through the Ugi Reaction. ChemPlusChem, 85(12), 2737–2741. Website

Rebordão, G., Palma, S. I. C. J., & Roque, A. C. A. (2020). Microfluidics in Gas Sensing and Artificial Olfaction. Sensors, 20, 1–22. PDF

Rodrigues, R., Palma, S. I. C. J., G. Correia, V., Padrão, I., Pais, J., Banza, M., Alves, C., Deuermeier, J., Martins, C., Costa, H. M. A., Ramou, E., Silva Pereira, C., & Roque, A. C. A. (2020). Sustainable plant polyesters as substrates for optical gas sensors. Materials Today Bio, 8. PDF

Roque, A. C. A., Pina, A. S., Azevedo, A. M., Aires-Barros, R., Jungbauer, A., Di Profio, G., Heng, J. Y. Y., Haigh, J., & Ottens, M. (2020). Anything but Conventional Chromatography Approaches in Bioseparation. Biotechnology Journal, 15(8), 1–8. Website

Santos, R. dos, Iria, I., Manuel, A. M., Leandro, A. P., Madeira, C. A. C., Goncalves, J., Carvalho, A. L., & Roque, A. C. A. (2020). Magnetic Precipitation: A New Platform for Protein Purification. Biotechnology Journal, 15(9), 1–11. Website

Pappas, C. G., Wijerathne, N., Sahoo, J. K., Jain, A., Kroiss, D., Sasselli, I. R., Pina, A. S., Lampel, A., & Ulijn, R. V. (2020). Spontaneous Aminolytic Cyclization and Self‐Assembly of Dipeptide Methyl Esters in Water. ChemSystemsChem, 2(5). Website

Book

Iranzo, O., & Roque, A. C. (2020). Peptide and Protein Engineering: From Concepts to Biotechnological Applications (O. Iranzo & A. C. Roque (eds.)). U.S.A: Humana Press.

2019

Barbosa, A. J. M., & Roque, A. C. A. (2019). Free Marine Natural Products Databases for Biotechnology and Bioengineering. Biotechnology Journal, 14(11), 1–8. Website

Batalha, I. L., Lychko, I., Branco, R. J. F., Iranzo, O., & Roque, A. C. A. (2019). β-Hairpins as peptidomimetics of human phosphoprotein-binding domains. Organic and Biomolecular Chemistry, 17(16), 3996–4004. Website

Carvalho, H. F., Branco, R. J. F., Leite, F. A. S., Matzapetakis, M., Roque, A. C. A., & Iranzo, O. (2019). Hydrolytic zinc metallopeptides using a computational multi-state design approach. Catalysis Science and Technology, 9(23), 6723–6736. Website

dos Santos, R., Figueiredo, C., Viecinski, A. C., Pina, A. S., Barbosa, A. J. M., & Roque, A. C. A. (2019). Designed affinity ligands to capture human serum albumin. Journal of Chromatography A, 1583, 88–97. Website

Esteves, C., Santos, G. M. C., Alves, C., Palma, S. I. C. J., Porteira, A. R., Filho, J., Costa, H. M. A., Alves, V. D., Morais Faustino, B. M., Ferreira, I., Gamboa, H., & Roque, A. C. A. (2019). Effect of film thickness in gelatin hybrid gels for artificial olfaction. Materials Today Bio, 1, 1–8. PDF Website

Fernandes, C. S. M., Pina, A. S., Barbosa, A. J. M., Padrão, I., Duarte, F., Teixeira, C. A. S., Alves, V., Gomes, P., Fernandes, T. G., Dias, A. M. G. C., & Roque, A. C. A. (2019). Affinity-Triggered Assemblies Based on a Designed Peptide–Peptide Affinity Pair. Biotechnology Journal, 14(11), 1–10. Website

Maugeri, G., Lychko, I., Sobral, R., & Roque, A. C. A. (2019). Identification and Antibiotic-Susceptibility Profiling of Infectious Bacterial Agents: A Review of Current and Future Trends. Biotechnology Journal, 14(1), 1–18. PDF Website

Reviewed conference papers

Pádua, A., Gruber, J., Gamboa, H., & Roque, A. C. (2019). Impact of Sensing Film’s Production Method on Classification Accuracy by Electronic Nose. Proceedings of the 12th International Joint Conference on Biomedical Engineering Systems and Technologies, 1, 56–64. PDF

Palma, S. I. C. J., Esteves, C., Padua, A. C. C. S., Alves, C. M., Santos, G. M. C., Costa, H. M. A., Dionisio, M., Gamboa, H., Gruber, J., & Roque, A. C. A. (2019). Enhanced Gas Sensing with Soft Functional Materials. IEEE International Symposium on Olfaction and Electronic Nose (ISOEN), 1–3. PDF

Santos, G., Alves, C., Pádua, A. C., Palma, S., Gamboa, H., & Roque, A. C. (2019). An Optimized E-nose for Efficient Bolatile Sensing and Discrimination. Proceedings of the 12th International Joint Conference on Biomedical Engineering Systems and Technologies, 1, 36–46. PDF

Roque, A., Fred, A., & Gamboa, H. (2019). Foreword. BIODEVICES 2019 – 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019, IX–XI. Website

2018

Barbosa, A. J. M., Oliveira, A. R., & Roque, A. C. A. (2018). Protein- and Peptide-Based Biosensors in Artificial Olfaction. Trends in Biotechnology, 36(12), 1244–1258. PDF Website

Palma, S. I. C. J., Traguedo, A. P., Porteira, A. R., Frias, M. J., Gamboa, H., & Roque, A. C. A. (2018). Machine learning for the meta-analyses of microbial pathogens’ volatile signatures. Scientific Reports, 8(3360), 1–15. PDF

Semeano, A. T. S., Maffei, D. F., Palma, S., Li, R. W. C., Franco, B. D. G. M., Roque, A. C. A., & Gruber, J. (2018). Tilapia fish microbial spoilage monitored by a single optical gas sensor. Food Control, 89, 72–76. PDF Website

Giancristofaro, A., Barbosa, A. J. M., Ammazzalorso, A., Amoia, P., De Filippis, B., Fantacuzzi, M., Giampietro, L., Maccallini, C., & Amoroso, R. (2018). Discovery of new FXR agonists based on 6-ECDCA binding properties by virtual screening and molecular docking. MedChemComm, 9(10), 1630–1638. Website

Reviewed conference papers

Pádua, A., Osório, D., Rodrigues, J., Santos, G., Porteira, A., Palma, S., Roque, A. C., & Gamboa, H. (2018). Scalable and Easy-to-use System Architecture for Electronic Noses. Proceedings of the 11th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2018), 1(Biostec), 179–186. PDF

Pádua, A. C., Palma, S., Gruber, J., Gamboa, H., & Roque, A. C. (2018). Design and Evolution of an Opto-electronic Device for VOCs Detection. Proceedings of the 11th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2018), 1(Biostec), 48–55. PDF Website

Book chapters

Fernandes, C. S. M., Teixeira, G. D. G., Iranzo, O., & Roque, A. C. A. (2018). Engineered Protein Variants for Bioconjugation. In B. Sarmento & J. Neves (Eds.), Biomedical Applications of Functionalized Nanomaterials: Concepts, Development and Clinical Translation (1st ed., pp. 105–138). Elsevier Inc.

2017

Palma, S. I. C. J., & Roque, A. C. A. (2017). Hybrid Magnetic-Polymeric Iron Oxide Nanoprobes for Magnetic Resonance Imaging. Journal of Nanoscience and Nanotechnology, 17(7), 4410–4431. Website

Hussain, Abid, Semeano, A. T. S., Palma, S. I. C. J., Pina, A. S., Almeida, J., Medrado, B. F., Pádua, A. C. C. S., Carvalho, A. L., Dionísio, M., Li, R. W. C., Gamboa, H., Ulijn, R. V., Gruber, J., & Roque, A. C. A. (2017). Tunable Gas Sensing Gels by Cooperative Assembly. Advanced Functional Materials, 27, 1–9. PDF Website

Fernandes, C. S. M., Pina, A. S., Batalha, Í. L., & Roque, A. C. A. (2017). Magnetic fishing of recombinant green fluorescent proteins and tagged proteins with designed synthetic ligands. Separation Science and Technology (Philadelphia), 52(18), 2907–2915. Website

dos Santos, R., Carvalho, A. L., & Roque, A. C. A. (2017). Renaissance of protein crystallization and precipitation in biopharmaceuticals purification. Biotechnology Advances, 35(1), 41–50. Website

Dias, Ana M.G.C., & Roque, A. C. A. (2017). The Future of Protein Scaffolds as Affinity Reagents for Purification. Biotechnology and Bioengineering, 114(3), 481–491. Website

Book chapters

Carvalho, H. F., Barbosa, A. J. M., Roque, A. C. A., Iranzo, O., & Branco, R. J. F. (2017). Integration of Molecular Dynamics Based Predictions into the Optimization of De Novo Protein Designs: Limitations and Benefits Henrique. In I. Samish (Ed.), Computational Protein Design (Vol. 1529, pp. 181–201). New York: Humana Press.

2016

Batalha, I., & Roque, A. (2016). Petasis-Ugi ligands: New affinity tools for the enrichment of phosphorylated peptides. Journal of Chromatography B, 1031, 86–93. Website

Batalha, Iris L., Zhou, H., Lilley, K., Lowe, C. R., & Roque, A. C. A. (2016). Mimicking nature: Phosphopeptide enrichment using combinatorial libraries of affinity ligands. Journal of Chromatography A, 1457, 76–87. Website

Dias, A. M.G.C., Dos Santos, R., Iranzo, O., & Roque, A. C. A. (2016). Affinity adsorbents for proline-rich peptide sequences: A new role for WW domains. RSC Advances, 6(73), 68979–68988. Website

Fernandes, C. S. M., Barbosa, I., Castro, R., Pina, A. S., Coroadinha, A. S., Barbas, A., & Roque, A. C. A. (2016). Retroviral particles are effectively purified on an affinity matrix containing peptides selected by phage-display. Biotechnology Journal, 11(12), 1513–1524. Website

Fernandes, C. S. M., Castro, R., Coroadinha, A. S., & Roque, A. C. A. (2016). Small synthetic ligands for the enrichment of viral particles pseudotyped with amphotropic murine leukemia virus envelope. Journal of Chromatography A, 1438, 160–170. Website

Fernandes, C. S. M., dos Santos, R., Ottengy, S., Viecinski, A. C., Béhar, G., Mouratou, B., Pecorari, F., & Roque, A. C. A. (2016). Affitins for protein purification by affinity magnetic fishing. Journal of Chromatography A, 1457, 50–58. Website

Palma, S. I. C. J., Fernandes, A. R., & Roque, A. C. A. (2016). An affinity triggered MRI nanoprobe for pH-dependent cell labeling. RSC Advances, 6, 113503–113512. Website

Pina, Ana Sofia, Carvalho, S., Dias, A. M. G. C., Guilherme, M., Pereira, A. S., Caraça, L. T., Coroadinha, A. S., Lowe, C. R., & Roque, A. C. A. (2016). Tryptophan tags and de novo designed complementary affinity ligands for the expression and purification of recombinant proteins. Journal of Chromatography A, 1472, 55–65. Website

Book chapters

Batalha, Í. L., & Roque, A. C. A. (2016). Phosphopeptide Enrichment Using Various Magnetic Nanocomposites: An Overview. In L. von Stechow (Ed.), Phospho-Proteomics: Methods and Protocols (2nd ed., Vol. 1355, pp. 193–209). Humana Press. Website

2015

Pina, Ana Sofia, Dias, A. M. G. C., Ustok, F. I., El Khoury, G., Fernandes, C. S. M., Branco, R. J. F., Lowe, C. R., & Roque, A. C. A. (2015). Mild and cost-effective green fluorescent protein purification employing small synthetic ligands. Journal of Chromatography A, 1418, 83–93. Website

Palma, S. I. C. J., Marciello, M., Carvalho, A., Veintemillas-Verdaguer, S., Morales, M. del P., & Roque, A. C. A. (2015). Effects of phase transfer ligands on monodisperse iron oxide magnetic nanoparticles. Journal of Colloid and Interface Science, 437(1), 147–155.Website

Palma, S. I. C. J., Rodrigues, C. A. V., Carvalho, A., Morales, M. D. P., Freitas, F., Fernandes, A. R., Cabral, J. M. S., & Roque, A. C. A. (2015). A value-added exopolysaccharide as a coating agent for MRI nanoprobes. Nanoscale, 7(34), 14272–14283. Website

Palma, S. I. C. J., Carvalho, A., Silva, J., Martins, P., Marciello, M., Fernandes, A. R., del Puerto Morales, M., & Roque, A. C. A. (2015). Covalent coupling of gum arabic onto superparamagnetic iron oxide nanoparticles for MRI cell labeling: physicochemical and in vitro characterization. Contrast Media and Molecular Imaging, 10(4), 320–328. Website

Fernandes, C. S. M., Goncçalves, B., Sousa, M., Martins, D. L., Barroso, T., Pina, A. S., Peixoto, C., Aguiar-Ricardo, A., & Roque, A. C. A. (2015). Biobased Monoliths for Adenovirus Purification. ACS Applied Materials and Interfaces, 7(12), 6605–6612. Website

Dias, Ana M.G.C., Iranzo, O., & Roque, A. C. A. (2015). An in silico and chemical approach towards small protein production and application in phosphoproteomics. RSC Adv., 5, 19743–19751. Website

Carvalho, H. F., Roque, A. C. A., Iranzo, O., & Branco, R. J. F. (2015). Comparison of the Internal Dynamics of Metalloproteases Provides New Insights on Their Function and Evolution. PLoS ONE, 10(9), 1–22. Website

Alves, B. M., Borlido, L., Rosa, S. A. S. L., Silva, M. F. F., Aires-Barros, M. R., Roque, A. C. A., & Azevedo, A. M. (2015). Purification of human antibodies from animal cell cultures using gum arabic coated magnetic particles. Journal of Chemical Technology and Biotechnology, 90(5), 838–846. Website

2014

Barroso, T., Branco, R. J. F., Aguiar-Ricardo, A., & Roque, A. C. A. (2014). Structural evaluation of an alternative Protein A biomimetic ligand for antibody purification. Journal of Computer-Aided Molecular Design, 28(1), 25–34. Website

Barroso, T., Casimiro, T., Ferraria, A. M., Mattioli, F., Aguiar-Ricardo, A., & Roque, A. C. A. (2014). Hybrid Monoliths for Magnetically-Driven Protein Separations. Advanced Functional Materials, 24(28), 4528–4541. Website

Dhadge, V. L., Hussain, A., Azevedo, A. M., Aires-Barros, R., & Roque, A. C. A. (2014). Boronic acid-modified magnetic materials for antibody purification. Journal of the Royal Society Interface, 11(91). Website

Dhadge, V. L., Morgado, P. I., Freitas, F., Reis, M. A., Azevedo, A., Aires-Barros, R., & Roque, A. C. A. (2014). An extracellular polymer at the interface of magnetic bioseparations. Journal of the Royal Society Interface, 11(100). Website

Dhadge, V. L., Rosa, S. A. S. L., Azevedo, A., Aires-Barros, R., & Roque, A. C. A. (2014). Magnetic aqueous two phase fishing: A hybrid process technology for antibody purification. Journal of Chromatography A, 1339, 59–64. Website

Fernandes, C. S. M., Pina, A. S., Dias, A. M. G. C., Branco, R. J. F., & Roque, A. C. A. (2014). A theoretical and experimental approach toward the development of affinity adsorbents for GFP and GFP-fusion proteins purification. Journal of Biotechnology, 186, 13–20. Website

Kadar, E., Batalha, Í. L., Fisher, A., & Roque, A. C. A. (2014). The interaction of polymer-coated magnetic nanoparticles with seawater. Science of the Total Environment, 487, 771–777. Website

Pina, Ana S., Batalha, Í. L., Fernandes, C. S. M., Aoki, M. A., & Roque, A. C. A. (2014). Exploring the potential of magnetic antimicrobial agents for water disinfection. Water Research, 66, 160–168. Website

Pina, Ana S., Guilherme, M., Pereira, A. S., Fernandes, C. S. F. M., Branco, R. J. F., El Khoury, G., Lowe, C. R., & Roque, A. C. A. (2014). A tailor-made “tag-receptor” affinity pair for the purification of fusion proteins. ChemBioChem, 15(10), 1423–1435. Website

Pina, Ana Sofia, Lowe, C. R., & Roque, A. C. A. (2014). Challenges and opportunities in the purification of recombinant tagged proteins. Biotechnology Advances, 32(2), 366–381. Website

Book chapters

Pina, A. S., Batalha, Í. L., & Roque, A. C. A. (2014). Affinity Tags in Protein Purification and Peptide Enrichment: An Overview. In N. E. Labrou (Ed.), Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods (2nd ed., Vol. 1129, pp. 147–168). Humana Press.

2013

Barroso, T., Hussain, A., Roque, A. C. A., & Aguiar-Ricardo, A. (2013). Functional monolithic platforms: Chromatographic tools for antibody purification Telma. Biotechnology Journal, 8(6), 671–681. Website

Barroso, T., Lourenço, A., Araújo, M., Bonifácio, V. D. B., Roque, A. C. A., & Aguiar-Ricardo, A. (2013). A green approach toward antibody purification: A sustainable biomimetic ligand for direct immobilization on (bio)polymeric supports. Journal of Molecular Recognition, 26(12), 662–671. Website

Borlido, L., Azevedo, A. M., Roque, A. C. A., & Aires-Barros, M. R. (2013). Magnetic separations in biotechnology. Biotechnology Advances, 31(8), 1374–1385. Website

Borlido, Luís, Moura, L., Azevedo, A. M., Roque, A. C. A., Aires-Barros, M. R., & Farinha, J. P. S. (2013). Stimuli-Responsive magnetic nanoparticles for monoclonal antibody purification. Biotechnology Journal, 8(6), 709–717. Website

Cerff, M., Scholz, A., Franzreb, M., Batalha, I. L., Roque, A. C. A., & Posten, C. (2013). In situ magnetic separation of antibody fragments from Escherichia coli in complex media. BMC Biotechnology, 13(44). Website

2012

Sandu, I. C. A., Roque, A. C. A., Matteini, P., Schäfer, S., Agati, G., Correia, C. R., & Viana, J. F. F. P. (2012). Fluorescence Recognition of Proteinaceous Binders in Works of Art by a Novel Integrated System of Investigation. Microscopy Research and Technique, 75(3), 316–324. Website

Sandu, I. C. A., Schäfer, S., Magrini, D., Bracci, S., & Roque, C. A. (2012). Cross-section and Staining-Based Techniques for Investigating Organic ,Materials in Painted and Polychrome Works of Art: A Review. Microscopy and Microanalysis, 18(4), 860–875. Website

Santana, S. D. F., Dhadge, V. L., & Roque, A. C. A. (2012). Dextran-Coated Magnetic Supports Modified with a Biomimetic Ligand for IgG Purification. ACS Applied Materials and Interfaces, 4(11), 5907–5914. Website

Santana, S. D. F., Pina, A. S., & Roque, A. C. A. (2012). Immobilization of enterokinase on magnetic supports for the cleavage of fusion proteins. Journal of Biotechnology, 161(3), 378–382. Website

Branco, R. J. F., Dias, A. M. G. C., & Roque, A. C. A. (2012). Understanding the molecular recognition between antibody fragments and protein A biomimetic ligand. Journal of Chromatography A, 1244, 106–115. Website

Cardoso, M. M., Peça, I. N., & Roque, A. C. A. (2012). Antibody-Conjugated Nanoparticles for Therapeutic Applications. Current Medicinal Chemistry, 19(19), 3103–3127. Website

Borlido, L., Azevedo, A. M., Sousa, A. G., Oliveira, P. H., Roque, A. C. A., & Aires-Barros, M. R. (2012). Fishing human monoclonal antibodies from a CHO cell supernatant with boronic acid magnetic particles. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 903, 163–170. Website

Batalha, Iris L., Lowe, C. R., & Roque, A. C. A. (2012). Platforms for enrichment of phosphorylated proteins and peptides in proteomics. Trends in Biotechnology, 30(2), 100–110. Website

Barroso, T., Roque, A. C. A., & Aguiar-Ricardo, A. (2012). Bioinspired and sustainable chitosan-based monoliths for antibody capture and release. RSC Advances, 2, 11285–11294. Website

Reviewed conference papers

Ataíde, F., Azevedo, C., Clemente, J. J., Cunha, A. E., Freitas, F., Reis, M. A. M., Roque, A. C. A., & Oliveira, R. (2012). Analysis of oxygen transport enhancement by function- alized magnetic nanoparticles (FMP) in bioprocesses. New Biotechnology, 29S(September), S75. Website

2011

Borlido, L., Azevedo, A. M., Roque, A. C. A., & Aires-Barros, M. R. (2011). Potential of boronic acid functionalized magnetic particles in the adsorption of human antibodies under mammalian cell culture conditions. Journal of Chromatography A, 1218(43), 7821–7827. Website

Dias, A. M.G.C., Hussain, A., Marcos, A. S., & Roque, A. C. A. (2011). A biotechnological perspective on the application of iron oxide magnetic colloids modified with polysaccharides. Biotechnology Advances, 29, 142–155. Website

2010

Barroso, T., Temtem, M., Hussain, A., Aguiar-Ricardo, A., & Roque, A. C. A. (2010). Preparation and characterization of a cellulose affinity membrane for human immunoglobulin G (IgG) purification. Journal of Membrane Science, 348(1–2), 224–230.Website

Batalha, Iris L., Hussain, A., & Roque, A. C. A. (2010). Gum Arabic coated magnetic nanoparticles with affinity ligands specific for antibodies. Journal of Molecular Recognition, 23(5), 462–471. Website

Bicho, A., Peça, I. N., Roque, A. C. A., & Cardoso, M. M. (2010). Anti-CD8 conjugated nanoparticles to target mammalian cells expressing CD8. International Journal of Pharmaceutics, 399(1–2), 80–86. Website

Bicho, Ana, Roque, A. C. A., Cardoso, A. S., Domingos, P., & Batalha, Í. L. (2010). In vitro studies with mammalian cell lines and gum arabic-coated magnetic nanoparticles. Journal of Molecular Recognition, 23(6), 536–542. Website

Ferreira, I. M.P.L.V.O., Pinho, O., Monteiro, D., Faria, S., Cruz, S., Perreira, A., Roque, A. C., & Tavares, P. (2010). Short communication: Effect of kefir grains on proteolysis of major milk proteins. Journal of Dairy Science, 93(1), 27–31. Website

Pina, A. S., Lowe, C. R., & Roque, A. C. A. (2010). Comparison of Fluorescence Labelling Techniques for the Selection of Affinity Ligands from Solid-Phase Combinatorial Libraries. Separation Science and Technology, 45(15), 2187–2193. Website

Book chapters

Pina, A. S., Hussain, A., & Roque, A. C. A. (2010). An Historical Overview of Drug Discovery. In A. C. A. Roque (Ed.), Ligand-Macromolecular Interactions in Drug Discovery: Methods and Protocols (Vol. 572, Issue 1, pp. 3–12). Totowa, NJ: Humana Press.

Book

Roque, A. C. A. (2010). Ligand-Macromolecular interaction in Drug Discovery. In A. C. A. Roque (Ed.), Methods in Molecular Biology. U.S.A: Humana Press.

2009

Hussain, A., Pina, A. S., & Roque, A. C. A. (2009). Bio-recognition and detection using liquid crystals. Biosensors and Bioelectronics, 25(1), 1–8. Website

Pina, A. S., & Roque, A. C. A. (2009). Studies on the molecular recognition between bioactive peptides and angiotensin-converting enzyme. Journal of Molecular Recognition, 22(2), 162–168. Website

Roque, A. C.A., Bispo, S., Pinheiro, A. R. N., Antunes, J. M. A., Gonçalves, D., & Ferreira, H. A. (2009). Antibody immobilization on magnetic particles. Journal of Molecular Recognition, 22(2), 77–82. Website

Roque, Ana C.A., Bicho, A., Batalha, I. L., Cardoso, A. S., & Hussain, A. (2009). Biocompatible and bioactive gum Arabic coated iron oxide magnetic nanoparticles. Journal of Biotechnology, 144(4), 313–320. Website

2008

Roque, A. C.A., & Wilson, O. C. (2008). Adsorption of gum Arabic on bioceramic nanoparticles. Materials Science and Engineering C, 28(3), 443–447. Website

Book chapters

Roque, A. C. A., & Lowe, C. R. (2008). Affinity Chromatography: History, Perspectives, Limitations and Prospects. In M. Zachariou (Ed.), Affinity Chromatography: Methods in Molecular Biology (2nd ed., Vol. 421, pp. 1–21). Totowa, NJ: Humana Press.

Roque, A. C. A., & Lowe, C. R. (2008). Rationally Designed Ligands for Use in Affinity Chromatography: An Artificial Protein L. In M. Zachariou (Ed.), Affinity Chromatography: Methods in Molecular Biology (2nd ed., Vol. 421, Issue 1, pp. 93–109). Totowa, NJ: Humana Press.

2007

Roque, A. C.A., Silva, C. S. O., & Taipa, M. Â. (2007). Affinity-based methodologies and ligands for antibody purification: Advances and perspectives. Journal of Chromatography A, 1160, 44–55. Website

Ferreira, I. M.P.L.V.O., Eça, R., Pinho, O., Tavares, P., Pereira, A., & Roque, A. C. (2007). Development and Validation of an HPLC/UV Method for Quantification of Bioactive Peptides in Fermented Milks. Journal of Liquid Chromatography and Related Technologies, 30, 2139–2147. Website

Roque, A. C. A., Silva, C. S. O., & Taipa, M. Â. (2007). Affinity-based methodologies and ligands for antibody purification: Advances and perspectives. Journal of Chromatography A, 1160, 44–55. Website